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Oroboros Instruments oroboros oxygraph 2k chamber
Oroboros Oxygraph 2k Chamber, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oxygraph-2k+chamber/high+resolution+respirometry/pmc12950476-78-6-6
Average 86 stars, based on 1 article reviews
oroboros oxygraph 2k chamber - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Phospho-proteomics:

Article Title: A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice.
Article Snippet: Mitochondrial oxygen consumption was measured in Oxygraph-2k (OROBOROS Instruments, Innsbruck, Austria) chamber at 37 °C in Mir05 buffer (110 mM sucrose, 60 mM lactobionic acid, 20 mM taurine, 20 mM HEPES, 10 mM KH2PO4, 3 mM MgCl2, 0.5 mM EGTA, and 1 g/l fatty acid-free BSA, pH 7.1).

Article Title: TEFM regulates both transcription elongation and RNA processing in mitochondria.
Article Snippet: Mitochondrial oxygen consumption flux was measured at 37°C using 25–50 lg of crude mitochondria diluted in 2.1 ml mitochondrial respiration buffer (120 mM sucrose, 50 mM KCl, 20 mM Tris–HCl, 4 mM KH2PO4, 2 mM MgCl2, and 1 mM EGTA, pH 7) within an Oxygraph-2k chamber (Oroboros Instruments).

Article Title: Influence of a Hyperglycemic Microenvironment on a Diabetic Versus Healthy Rat Vascular Endothelium Reveals Distinguishable Mechanistic and Phenotypic Responses
Article Snippet: Depending on the protein content, the suspension volume was adjusted to have a final protein concentration of 125 μg protein/mL in the O2K Core: Oxygraph-2k chamber (Cat no: 10000-02, Oroboros Instruments).

Article Title: The detrimental effects and mechanisms of Orlistat in disrupting energy homeostasis and reproduction in Daphnia magna.
Article Snippet: Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades.. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces.. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL.

Article Title: Excessive Exercise Elicits Poly (ADP-Ribose) Polymerase-1 Activation and Global Protein PARylation Driving Muscle Dysfunction and Performance Impairment.
Article Snippet: Next, 1.5mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Article Title: In Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy
Article Snippet: In total, 2.5 to 3 million cells were added to each 2 mL Oxygraph-2k chamber (Oroboros Instruments, Innsbruck, Austria).

Article Title: Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure
Article Snippet: The samples were then placed into the Oxygraph-2k chamber (Oroboros instruments, Innsbruck, Austria) containing buffer R+BSA and a Clark electrode to analyze non-phosphorylating respiration and oxidative phosphorylation using a multiple substrate-uncoupler-inhibitor titration (SUIT) protocol.

Article Title: Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
Article Snippet: Next, 1.5 mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Titration:

Article Title: A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice.
Article Snippet: Mitochondrial oxygen consumption was measured in Oxygraph-2k (OROBOROS Instruments, Innsbruck, Austria) chamber at 37 °C in Mir05 buffer (110 mM sucrose, 60 mM lactobionic acid, 20 mM taurine, 20 mM HEPES, 10 mM KH2PO4, 3 mM MgCl2, 0.5 mM EGTA, and 1 g/l fatty acid-free BSA, pH 7.1).

Article Title: TEFM regulates both transcription elongation and RNA processing in mitochondria.
Article Snippet: Mitochondrial oxygen consumption flux was measured at 37°C using 25–50 lg of crude mitochondria diluted in 2.1 ml mitochondrial respiration buffer (120 mM sucrose, 50 mM KCl, 20 mM Tris–HCl, 4 mM KH2PO4, 2 mM MgCl2, and 1 mM EGTA, pH 7) within an Oxygraph-2k chamber (Oroboros Instruments).

Article Title: Influence of a Hyperglycemic Microenvironment on a Diabetic Versus Healthy Rat Vascular Endothelium Reveals Distinguishable Mechanistic and Phenotypic Responses
Article Snippet: Depending on the protein content, the suspension volume was adjusted to have a final protein concentration of 125 μg protein/mL in the O2K Core: Oxygraph-2k chamber (Cat no: 10000-02, Oroboros Instruments).

Article Title: The detrimental effects and mechanisms of Orlistat in disrupting energy homeostasis and reproduction in Daphnia magna.
Article Snippet: Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades.. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces.. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL.

Article Title: Excessive Exercise Elicits Poly (ADP-Ribose) Polymerase-1 Activation and Global Protein PARylation Driving Muscle Dysfunction and Performance Impairment.
Article Snippet: Next, 1.5mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Article Title: In Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy
Article Snippet: In total, 2.5 to 3 million cells were added to each 2 mL Oxygraph-2k chamber (Oroboros Instruments, Innsbruck, Austria).

Article Title: Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure
Article Snippet: The samples were then placed into the Oxygraph-2k chamber (Oroboros instruments, Innsbruck, Austria) containing buffer R+BSA and a Clark electrode to analyze non-phosphorylating respiration and oxidative phosphorylation using a multiple substrate-uncoupler-inhibitor titration (SUIT) protocol.

Article Title: Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
Article Snippet: Next, 1.5 mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Fluorescence:

Article Title: A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice.
Article Snippet: Mitochondrial oxygen consumption was measured in Oxygraph-2k (OROBOROS Instruments, Innsbruck, Austria) chamber at 37 °C in Mir05 buffer (110 mM sucrose, 60 mM lactobionic acid, 20 mM taurine, 20 mM HEPES, 10 mM KH2PO4, 3 mM MgCl2, 0.5 mM EGTA, and 1 g/l fatty acid-free BSA, pH 7.1).

Article Title: TEFM regulates both transcription elongation and RNA processing in mitochondria.
Article Snippet: Mitochondrial oxygen consumption flux was measured at 37°C using 25–50 lg of crude mitochondria diluted in 2.1 ml mitochondrial respiration buffer (120 mM sucrose, 50 mM KCl, 20 mM Tris–HCl, 4 mM KH2PO4, 2 mM MgCl2, and 1 mM EGTA, pH 7) within an Oxygraph-2k chamber (Oroboros Instruments).

Article Title: Influence of a Hyperglycemic Microenvironment on a Diabetic Versus Healthy Rat Vascular Endothelium Reveals Distinguishable Mechanistic and Phenotypic Responses
Article Snippet: Depending on the protein content, the suspension volume was adjusted to have a final protein concentration of 125 μg protein/mL in the O2K Core: Oxygraph-2k chamber (Cat no: 10000-02, Oroboros Instruments).

Article Title: The detrimental effects and mechanisms of Orlistat in disrupting energy homeostasis and reproduction in Daphnia magna.
Article Snippet: Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades.. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces.. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL.

Article Title: Excessive Exercise Elicits Poly (ADP-Ribose) Polymerase-1 Activation and Global Protein PARylation Driving Muscle Dysfunction and Performance Impairment.
Article Snippet: Next, 1.5mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Article Title: In Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy
Article Snippet: In total, 2.5 to 3 million cells were added to each 2 mL Oxygraph-2k chamber (Oroboros Instruments, Innsbruck, Austria).

Article Title: Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure
Article Snippet: The samples were then placed into the Oxygraph-2k chamber (Oroboros instruments, Innsbruck, Austria) containing buffer R+BSA and a Clark electrode to analyze non-phosphorylating respiration and oxidative phosphorylation using a multiple substrate-uncoupler-inhibitor titration (SUIT) protocol.

Article Title: Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
Article Snippet: Next, 1.5 mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Suspension:

Article Title: A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice.
Article Snippet: Mitochondrial oxygen consumption was measured in Oxygraph-2k (OROBOROS Instruments, Innsbruck, Austria) chamber at 37 °C in Mir05 buffer (110 mM sucrose, 60 mM lactobionic acid, 20 mM taurine, 20 mM HEPES, 10 mM KH2PO4, 3 mM MgCl2, 0.5 mM EGTA, and 1 g/l fatty acid-free BSA, pH 7.1).

Article Title: TEFM regulates both transcription elongation and RNA processing in mitochondria.
Article Snippet: Mitochondrial oxygen consumption flux was measured at 37°C using 25–50 lg of crude mitochondria diluted in 2.1 ml mitochondrial respiration buffer (120 mM sucrose, 50 mM KCl, 20 mM Tris–HCl, 4 mM KH2PO4, 2 mM MgCl2, and 1 mM EGTA, pH 7) within an Oxygraph-2k chamber (Oroboros Instruments).

Article Title: Influence of a Hyperglycemic Microenvironment on a Diabetic Versus Healthy Rat Vascular Endothelium Reveals Distinguishable Mechanistic and Phenotypic Responses
Article Snippet: Depending on the protein content, the suspension volume was adjusted to have a final protein concentration of 125 μg protein/mL in the O2K Core: Oxygraph-2k chamber (Cat no: 10000-02, Oroboros Instruments).

Article Title: The detrimental effects and mechanisms of Orlistat in disrupting energy homeostasis and reproduction in Daphnia magna.
Article Snippet: Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades.. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces.. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL.

Article Title: Excessive Exercise Elicits Poly (ADP-Ribose) Polymerase-1 Activation and Global Protein PARylation Driving Muscle Dysfunction and Performance Impairment.
Article Snippet: Next, 1.5mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Article Title: In Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy
Article Snippet: In total, 2.5 to 3 million cells were added to each 2 mL Oxygraph-2k chamber (Oroboros Instruments, Innsbruck, Austria).

Article Title: Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure
Article Snippet: The samples were then placed into the Oxygraph-2k chamber (Oroboros instruments, Innsbruck, Austria) containing buffer R+BSA and a Clark electrode to analyze non-phosphorylating respiration and oxidative phosphorylation using a multiple substrate-uncoupler-inhibitor titration (SUIT) protocol.

Article Title: Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
Article Snippet: Next, 1.5 mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Protein Concentration:

Article Title: A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice.
Article Snippet: Mitochondrial oxygen consumption was measured in Oxygraph-2k (OROBOROS Instruments, Innsbruck, Austria) chamber at 37 °C in Mir05 buffer (110 mM sucrose, 60 mM lactobionic acid, 20 mM taurine, 20 mM HEPES, 10 mM KH2PO4, 3 mM MgCl2, 0.5 mM EGTA, and 1 g/l fatty acid-free BSA, pH 7.1).

Article Title: TEFM regulates both transcription elongation and RNA processing in mitochondria.
Article Snippet: Mitochondrial oxygen consumption flux was measured at 37°C using 25–50 lg of crude mitochondria diluted in 2.1 ml mitochondrial respiration buffer (120 mM sucrose, 50 mM KCl, 20 mM Tris–HCl, 4 mM KH2PO4, 2 mM MgCl2, and 1 mM EGTA, pH 7) within an Oxygraph-2k chamber (Oroboros Instruments).

Article Title: Influence of a Hyperglycemic Microenvironment on a Diabetic Versus Healthy Rat Vascular Endothelium Reveals Distinguishable Mechanistic and Phenotypic Responses
Article Snippet: Depending on the protein content, the suspension volume was adjusted to have a final protein concentration of 125 μg protein/mL in the O2K Core: Oxygraph-2k chamber (Cat no: 10000-02, Oroboros Instruments).

Article Title: The detrimental effects and mechanisms of Orlistat in disrupting energy homeostasis and reproduction in Daphnia magna.
Article Snippet: Orlistat (ORL) has been employed as an anti-obesity pharmaceutical for several decades.. Given its low absorption rate, the majority of administered ORL is excreted into the environment with feces.. It is crucial to collect scientific information regarding the possible ecological risks associated with ORL.

Article Title: Excessive Exercise Elicits Poly (ADP-Ribose) Polymerase-1 Activation and Global Protein PARylation Driving Muscle Dysfunction and Performance Impairment.
Article Snippet: Next, 1.5mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.

Article Title: In Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy
Article Snippet: In total, 2.5 to 3 million cells were added to each 2 mL Oxygraph-2k chamber (Oroboros Instruments, Innsbruck, Austria).

Article Title: Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure
Article Snippet: The samples were then placed into the Oxygraph-2k chamber (Oroboros instruments, Innsbruck, Austria) containing buffer R+BSA and a Clark electrode to analyze non-phosphorylating respiration and oxidative phosphorylation using a multiple substrate-uncoupler-inhibitor titration (SUIT) protocol.

Article Title: Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
Article Snippet: Next, 1.5 mg was put into an Oxygraph-2k chamber (Oroboros Instruments Corporation, Innsbruck, Austria) with 2 mL of MIR buffer.



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